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11 Efforts in Vascular Tissue Engineering Several strategies have been attempted in the construction of autologous smallcaliber vascular grafts The earliest efforts in vascular tissue engineering occurred in the 1980s, when Weinberg and co-workers developed methods to grow arteries by seeding vascular cells onto preformed, tubular collagen gel constructs [Weinberg and Bell, 1986] Several variations of this collagen gel-based approach have been explored with varying rates of success [Kanda et al, 1993; L Heureux et al, 1993; Hirai et al, 1994; Kanda and Matsuda, 1994; Tranquillo et al, 1996; Barocas et al, 1998; Girton et al, 1999] Yet, collagen gel-based constructs can typically only withstand burst pressures of 10 300 mmHg, which is not acceptable for vascular replacement in the coronary system [Girton et al, 2000].

Acellular collagenous grafts have also been investigated as an approach to arterial replacement by many groups [Badylak et al, 1989; Sandusky et al, 1992, 1995; Lantz et al, 1993; Hiles et al, 1995; Wilson et al, 1995; Inoue et al, 1996; Huynh et al, 1999; Roeder et al, 1999] The group led by Huynh [Huynh et al, 1999] utilized tubes of submucosal collagen derived from porcine small intestine These tubes were coated with bovine brillar collagen and then complexed with heparin-benzalkonium chloride to reduce the propensity for thrombus formation Although this acellular porcine collagen conduit demonstrated impressive mechanical strength, these types of grafts still possess considerable drawbacks The potential in ammatory immune response to vascular grafts composed of animal collagens remains largely unknown.

Additionally, acellular constructs lack an antithrombogenic endothelium and would have to acquire such an endothelial cell layer after implantation Endothelial cell recruitment from surrounding tissue is known to be poor in humans [Nerem and Seliktar, 2001] The rst completely biological vascular graft was reported in 1998 by L Heureux and colleagues Their approach was to layer continuous sheets of broblasts and human umbilical smooth muscle cells around a central mandrel to form tubular vessels [L Heureux et al, 1998] After a 6- to 13-week culture period, the inner lumen of the tubular construct was seeded with endothelial cells [L Heureux et al, 1998] Although these arti cial vessels were able to withstand high pressures, displaying rupture strengths greater than 2000 mmHg, their strength is derived primarily from the adventitial layer rather than the medial layer, which carries.

If not, one can either start with (24 1) or 23 calls, then with (24 2) or 22 calls, and so on until a point is reached at which all the connection attempts are successful Alternatively, one can start with only one connection request, then increase it to two, three, four, and so on, and monitor the status of the connection requests in Hammer s monitor window to determine the maximum number (eg, n1 ) of calls that can be started simultaneously resulting in successful connections A owchart describing various phases of step 1 is presented in Figure B-4 Step 2: Once step 1 is completed, one must determine experimentally the intercall-burst interval that allows establishment of connections on all 24 channels for one set of T1 connection to the IP-PSTN GWs from the Hammer tester.

Treatment of Essential Hypertension Adherence to Treatment 356 Summary 356 CONCLUSION 356 REFERENCES 357

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Table 2-2

tain to some of the employees recent personal experiences at the time of check-in or checkout may initiate some opportunities for discussion. Training continues with a list of empowerment policy standards, which describe the authority and responsibility that are included in their job description. Employee-manager dialogue about these standards will help clarify employee understanding and concerns and identify manager communication issues. The manager will want to demonstrate and have employees go over the use of empowerment policy standards. Managers will also want to have follow-up training sessions with review of employee performance and opportunities for employee feedback.

x2 = 138.80, p = .22, df = 127, RMSEA = .020, 90% UL = .039, AIC = 115.20 German Sample Trust Straightforwardness Altruism Compliance Modesty Tender-mindedness .38 .09 .72 .41 .00 .23 .00 .32 .15 .20 1.00 .19 .24 .00 .25 .06 .04 .17 .03 .29 .14 .22 .29 .03 .62 .59 .13 .39 .00 .58 .73 .71 .61 .73 .67 .80