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Reagents and Materials Sterile Ethoxyresoru n, 1 mM stock in dimethyl sulfoxide (DMSO) Dicumarol, 2 mM stock in 75 mM NaOH 3-Methylcholanthrene (3MC), 10 mM stock in DMSO CHCM (See Section 2.6) CHCM (See Section 2.6), without phenol red Nonsterile 96-Well plate 24-Well plate 0.1 M NaOH -Glucuronidase, 1600 U/ml in 0.1 M sodium acetate buffer Resoru n, 1 mM stock in DMSO Spectro uorometer Protocol (a) This protocol is based on hepatocyte cultures in 6-well plates. Volumes given are on a per well basis, and can be easily scaled for the use with cultures of various dimensions. (b) At the time point indicated by the particular experiment, remove culture medium from hepatocyte cultures and add CHCM containing 2 M 3MC. Incubate cultures in 3MC-containing medium for 48 72 h, adding fresh medium with 3MC every 24 h, to induce suf ciently the expression of CYP1A. Alternatively, a 5 M concentration of -naptho avone can be used in place of 3MC to induce CYP1A expression by rat hepatocytes. For experiments requiring the induction of CYP2B expression, 1 mM phenobarbital can be used. (c) Aspirate medium and carefully wash wells with CHCM without phenol red. Add 750 l solution of 5 M EROD and 10 M dicumarol in CHCM without Phenol Red. Incubate for 20 min at 37 C. Dicumarol is a competitive inhibitor of cytosolic oxidoreductases and prevents diaphorase-mediated metabolism of resoru n. (d) Transfer 750 l aliquot to 24-well plate. Add 150 l 1600 U/ml -glucuronidase solution and incubate at 37 C for a minimum of 2 h (maximum overnight).

Many comparative psychologists participate in these The International Society for Comparative psychology, founded in 1983, has great possibilities that have not yet been realized Soul-Searching Comparative psychologists have often assessed the state of their discipline and often criticized the directions taken Three major articles stand out In his famous The Snark Was a Boojum, mentioned earlier, Beach (1950) argued that comparative psychology had begun as the study of a wide range of topics in a wide range of species but had degenerated into the study of learning in rats He stressed the need for a resurrection of the breadth that had earlier characterized the eld Although Beach s analysis was awed (Dewsbury, 1998), it was quite in uential In the second major critique, Hodos and Campbell (1969) criticized comparative psychologists perspective on evolutionary history.

Table 9.1 Design system asymmetric diode string parameterized cell HBM and MM ESD results SiGeC asymmetric diode string 5:1 number of cathodes 2 4 6 8 10 HBM failure voltage (V) 2300 3500 4800 6000 7200 MM failure voltage (V) 240 390 510 720 750

Many of the guidelines are really common sense except that we normally would not consider them necessary in a mixed-language environment. For example, because we now have to consider how other components will interact with our component even when they are developed in different languages, we should try to avoid using more global/publicly visible constructs than necessary. Similarly, we should place even more emphasis on programming to interface, which is the informal name for the general technique of exporting public interfaces and using them rather than directly referencing the class that implements a particular publicly accessible method. In this case, using the interface makes it easier to reimplement the code later on using a different programming language or to support multiple implementations at the same time. Although both of these examples are good practices to follow in any environment, we would usually not apply them across code written in different programming languages because different programming languages do not typically interoperate this way outside this platform. When we create our Microsoft .NET Framework-based components, we should be aware of the execution issues we discussed in Section 14.2.3, particularly the multitasking considerations. Earlier, we mentioned that the Application Domain acts like a virtual machine for the components executing within it; now we can also emphasize that a given thread of control is active within only one Application Domain at a time. As a component executes, a given thread of control can travel across domains, and a given domain can have multiple threads. While those details sound like they should be discussed in the next section, which considers deployment details, we mention these facts here to emphasize that the threading model is somewhat different from most traditional scenarios, and therefore must be addressed very carefully during the software design and implementation processes.

10 X Liu, Q Dai, and Q Wu, An improved resource reservation algorithm for IEEE 802153, paper presented at the IEEE ICME 06, Toronto, Canada, July 2006, pp 589 592 11 R Zeng and G S Kuo, A novel scheduling scheme and MAC enhancements for IEEE 802153 high-rate WPAN, paper presented at the IEEE WCNC 05, Vol 4, New Orleans, LA, Mar 2005, pp 2478 2483 12 S H Rhee, K Chung, Y Kim, W Yoon, and K S Chang, An application-aware MAC scheme for IEEE 802153 high-rate WPAN, paper presented at the IEEE WCNC 04, Vol 2, Atlanta, GA, Mar 2004, pp 1018 1023 13 B-S Kim, Y Fang, and T F Wong, Rate-adaptive MAC protocol in high-rate personal area networks, paper presented at the IEEE WCNC 04, Vol 3, Atlanta, GA, Mar 2004, pp 1394 1399 14 D Tarchi, R.